Regulatory

Part:BBa_K1895002

Designed by: iGEM16_Newcastle   Group: iGEM16_Newcastle   (2016-09-06)


dnaK Promoter

This is the dnaK promoter from E. coli K-12.

Usage and Biology

DnaK is a heat shock protein. It's role is that of a protein chaperone, it binds to proteins that have misfolded due to the stress. These proteins are then refolded in an HSP-mediated process or degraded (Arsène F, et al., 2000). Like other members of the Hsp70 family, dnaK in E. coli is upregulated by heat stress (Ritossa, F., 1996). Consequently its promoter must be stronger during heat shock to allow for overexpression. Although DnaK itself acts to shut off the heat shock response (Tilly, K. et al., 1983) we feel its promoter is a viable alternative heat shock promoter to the current registry entry, htpG (BBa_J45504).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma32
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
Parameters
None